Human milk bank (HMB) services are an essential component of a breastfeeding-friendly health system. HMB services give at-risk infants, such as those born pre-term or low birthweight, access to the multiple benefits of breastmilk when they need it most. Alive & Thrive (A&T) and other partners supported the Da Nang Department of Health and Tu Du Hospital to set up the first two HMBs in Viet Nam. The HMB at Da Nang Hospital for Women and Children (DNHWC) was established in 2016, while the HMB at Tu Du Hospital opened in 2019. A&T has developed this brief in order to share information, resources, and lessons learned from the experience of setting up the first HMBs in Viet Nam.